By M. Caliskan
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Extra info for Analysis of Genetic Variation in Animals
This study included analyses of heterozygosity, inbreeding, the Hardy-Weinberg equilibrium test and breeds relationship. 5 software (Felsenstein, 1989). We collected fresh blood samples from 190 randomly chosen individuals and the isolation of genomic DNA was performed with the Wizard Genomic DNA Extraction Kit (Promega). The amplification of the microsatellite loci (TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA23, ETH3, ETH225, and BM1824) was carried out using StockMarks® for Cattle Bovine Genotyping Kit (AppliedBiosystems).
Microsatellite VHL20 HTG4 AHT4 HMS7 HTG6 AHT5 HMS6 ASB2 HTG10 HTG7 HMS3 HMS2 Thoroughbred 6 7 6 5 9 8 6 11 12 7 10 5 Arabian 8 3 8 5 6 7 7 8 11 7 8 9 Romanian Sport Horse 8 4 7 6 6 5 5 8 7 3 7 8 Table 1. , 2008). , 2008). 122 Table 2. , 2008). 105775 Table 3. , 2008). Fig. 1. Phylogenetic tree constructed based on Reynolds’ genetic distance by UPGMA method. , 2008). 8% for the Arabian-Hucul pair. 194 (Table 3). , 2008). The phylogenetic tree obtained by the UPGMA method shows an early and clear divergence of the Hucul breed in comparison to the other three breeds analyzed (Figure 1).
2009). Fst estimates compared in pairs and the Cavalli-Sforza’s chord distance (Cavali-Sforza and Edwards, 1967) have been presented in Table 9. 219, for Pietrain-Wild Boar pair. , 2009). This dendrogram presents the phylogenetic relationships among the analyzed Romanian swine populations. 146 Table 9. , 2009). As shown in Figure 3, with a bootstrap value of 100% Mangalitsa is the closest breed to the Wild Boar. , 2009). This study was the first based on microsatellite markers for the genetic characterization of swine populations from Romania and the data obtained confirmed the ancient origin of the Mangalitsa.
Analysis of Genetic Variation in Animals by M. Caliskan